Probiotic enema protects intestinal mucosa and alters plasma diamine oxidase activity among calves with diarrhea

Abstract

Diamine oxidase (dAo) (alternatively histaminases,in under the old denomination ), as a natural enzyme present in high levels, promotes the integrity and maturation of the small intestinal mucosa. In the present study, we sought to investigate whether the plasma levels of diamine oxidase activity denote mucosal injury during diarrhea among calves, and we attempted to use rectal enema probiotic therapy against it. Upon arrival at a private and commercial milk-fed calf facility, calves were subjected to scoring on fecal consistency on a scale from 0 to 3. Calves exhibiting a fecal score of 2 (loose feces)-or 3 (watery feces) were enrolled as diarrheic. As solely calves with diarrhea were enrolled. All diarrheic calves received rectal enema multi strain probiotic treatment (Farm Rumin Probiotic Powder). The mean dAo levels (ng/mL) among diarrheic calves before and after probiotic enema treatment were detected as 8.48±1.67 and 28.06±3.51, respectively, presenting statistically significant alterations (p<0.001). In summary, it should not be unwise to draw a preliminary conclusion that plasma dAo activity was decreased in response to intestinal mucosal injury in relationship with diarrhea, which was reversed with rectal enema probiotic treatment for 10 days, reflecting a feed-back regulation of dAo activity connected with mucosal recovery, as was proposed.