Analysis of some interleukins and leukogram in ISA Brown cocks experimentally infected with Salmonella gallinarum

Abstract

This research aimed to investigate the profile of IL- 1β, INFγ, and leukogram in cocks experimentally infected with Salmonella gallinarum. A total of 40 cocks, infected and control, 20 birds each were used for this study. Each bird in the infected group was administered orally 1ml inoculum 9.0 x 108 CFU/ml Salmonella gallinarum. Blood samples were collected and analyzed for leukogram, and the harvested serum samples were analyzed for IL-Iβ and INF γ. There were significant differences (p < 0.05) in the mean absolute TWBC, heterophil and lymphocyte counts in the infected and the control cocks almost at levels. The highest values of TWBC (14.5 ± 0.60 x 109/1), heterophil ( 4.4 ± 0.31 x 109/1) and lymphocyte (10.4±0.55 x 109/1) counts were observed in the infected group on Day 21 (p.i.). There were significant differences (p < 0.05) in the mean serum IL-1β and IFN-γ concentrations in the infected and the control cocks on Day 4 (185±46.0 pg/ml) and Day 7 p.i. (2634± 342 pg/ml), respectively. The highest and lowest values for mean serum IL-1β concentration kwere observed to be 695±1.0 pg/ml and 185±46.0 pg/ml in the infected group on Days 14 and 7 p.i., while that of IFN γ was 4134±217 pg/ml and 2634 ± 342 pg/ml in the infected group on Days15 p.i. and cocks may play a vital role in driving higher immune responses during Salmonella gallinarum infection.