Effects of newly synthesized xanthene-3-ones on Pseudomonas aeruginosa – Experimental study on rats

Abstract

The need for new drugs that will have an antimicrobial effect is increasing every day. Xanthenes are cyclic, organic compounds of natural, semi-synthetic or synthetic origin, which in many studies have shown good antimicrobial, antioxidant, antiproliferative, antidiabetic, neuroprotective and many other biological effects and are interesting for further research. In the study, the antimicrobial activity of two newly synthesized xanthenic compounds was investigated: 2,6,7-trihydroxy-9-(2-hydroxy-5-bromophenyl)-3H-xanthene-3-one (Compound 1) and 2,6,7-trihydroxy-9-(3-bromophenyl)-3H-xanthene-3- one (Compound 2) to a wound infection caused by a bacterial strain Pseudomonas aeruginosa (ATCC 10145) on rats (n=36). The animals were divided into 6 groups. The first group was
treated with Compound 1 in the concentration of 0,626 mg/g, the second group with Compound 1 in the concentration of 1 mg/g, the third with Compound 2 in the concentration of 0,626 mg/g and the fourth by Compound 2 in the concentration of 1 mg/g. The fifth and sixth groups were comparative (gentamicin) and control (vaseline). The Compounds were prepared in the form of a dermal preparation with the concentrations of 0,626 mg/g and 1 mg/g and were applied to the wound every day in the amount of 1 mg. Swabs of the wounds were taken on 1.,2., 3., and 7. day after infection to determine whether there has been a reduction in the number of bacteria. The results showed that both Compounds ultimately led to the subsidence of the infection, but a statistically significant difference was only observed between the groups that were treated with Compound 2 in the concentration of 0,626 mg/g compared to the control group.